GSM4519179: HT29 ERB ChIP-Seq rep 3; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
ESR2
Cell type
Cell type Class
Digestive tract
Cell type
HT-29
Primary Tissue
Colon
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
colon
chip antibody
PPZ0506, lot no: A2, mouse monoclonal
cell line
HT29
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked with formaldehyde and nuclear chormatin fractions were separted and immunoprecipitated with ERβ anitboides. Magnetic beads were used to pull down the immunoprecipitated DNA and separted by column preparation. The libraries of ChIP DNA were prepared by using the kit NEB Next Ultra II DNA Library Prep Kit for Illumina following user instrcutions